We provide new data on the natural history and morphometrics of one of the least studied didelphid marsupial species, Lutreolina crassicaudata. Field work was conducted in central-eastern Argentina during two consecutive years. Juveniles and sub-adults were captured during summer and autumn. Reproduction showed seasonality with a continuous breeding period from spring to summer (September to February). The analysis of 17 scats belonging to 17 individuals showed mainly seeds and vegetal remains, as well as amphibians, birds and invertebrates. Sexual dimorphism in juveniles and sub-adults was registered for the first time, being males larger and heavier than females.

En este trabajo proporcionamos nuevos datos sobre la historia natural y morfometría de una de las especies de marsupiales didélfidos menos estudiadas, Lutreolina crassicaudata. El trabajo de campo se llevó a cabo en el centro-este de la Argentina durante dos años consecutivos. Los individuos juveniles y subadultos fueron capturados durante el verano y el otoño. La reproducción mostró estacionalidad, con un período de crianza continuo durante primavera y verano (septiembre a febrero). El análisis de 17 heces pertenecientes a 17 individuos presentó principalmente semillas y restos de vegetales, como así también anfibios, aves e invertebrados. Se registró dimorfismo sexual en las clases de edad juveniles y subadultos, siendo los machos más grandes y pesados que las hembras.

We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lec from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lec virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.

Dyad encounters between male and female adults of Akodon azarae (Fischer, 1829) were analyzed by means of observational techniques in a natural closure during the breeding and non-breeding seasons. The animals were held in observation during 21 days, with daily 15-minute recordings of interindividual separation distance, relative displacements, characteristics of the male-female interaction, copulation, and construction and exclusive or shared use of nests by each pair. The couples, which bred successfully, showed, on average, the longest separation distance between male and female allowed by the closure. During the first two weeks of gestation the females exhibited more displacements than their respective mates did. The male-pregnant female encounters were significantly more aggressive than those recorded between pairs which did not breed successfully. During the non-breeding season a shorter average distance between individuals and a frequent use of nests shared by the pair were recorded. The results obtained are discussed within the framework of the social system of A. azarae.